biotinylated peanut agglutinin (pna) (Vector Laboratories)
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Biotinylated Peanut Agglutinin (Pna), supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 95/100, based on 912 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/peanut+agglutinin/Biotinylated+Peanut+Agglutinin+(PNA)/custom%40b-1075%4042414656
Average 95 stars, based on 912 article reviews
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Incubation:Article Title: Epididymal epithelial cells facilitate NEU1 loading to modulate sperm α-2,6 sialylation, enhance maturation and motility Article Snippet: Blocking was conducted with 3% BSA (BIOFROXX) in permeabilization buffer at RT for 1 h with rotation. .. Primary antibodies (anti-NEU1 [1:100, GenScript], anti-NEU3 [1:1000, GenScript], Biotinylated Maackia Amurensis Lectin I [anti-MAL-I, 1:50, Vector Labs], Biotinylated Maackia Amurensis Lectin II [anti-MAL-II, 1:50, Vector Labs], biotinylated Sambucus Nigra Lectin [anti-SNA, 1:50, Vector Labs], biotinylated Wheat Germ Agglutinin [anti-WGA, 1:50, Vector Labs], Article Title: Overexpression of Tn antigen induces chronic pancreatitis in mice. Article Snippet: Proteins were then transferred onto a nitrocellulose membrane (Thermo Fisher). .. The membrane was blocked with 1× Carbo-Free Blocking Solution (Biozol) in TBS-T and incubated overnight with serum diluted 1:20 in TBS-T. Biotinylated lectins, including 10 μg/ml Vicia villosa lectin (VVA, B-1235; Vector Laboratories) and 10 μg/ml Article Title: Novel mutations in ZMYND15 are associated with male infertility with oligozoospermia/azoospermia. Article Snippet: Purpose This study aimed to identify the genetic causes of male infertility associated with oligozoospermia/azoospermia in two unrelated Chinese families.. Methods Whole-exome sequencing (WES) and Sanger sequencing were performed on peripheral blood samples from three infertile individuals with reduced sperm counts.. Semen analysis data were collected, and sperm morphology was evaluated using hematoxylin and eosin staining, along with transmission electron microscopy. Article Title: Epididymal epithelial cells facilitate NEU1 loading to modulate sperm α-2,6 sialylation, enhance maturation and motility. Article Snippet: Blocking was conducted with 3% BSA (BIOFROXX) in permeabilization buffer at RT for 1 h with rotation. .. Primary antibodies (anti-NEU1 [1:100, GenScript], anti-NEU3 [1:1000, GenScript], Biotinylated Maackia Amurensis Lectin I [anti-MAL-I, 1:50, Vector Labs], Biotinylated Maackia Amurensis Lectin II [anti-MAL-II, 1:50, Vector Labs], biotinylated Sambucus Nigra Lectin [anti-SNA, 1:50, Vector Labs], biotinylated Wheat Germ Agglutinin [anti-WGA, 1:50, Vector Labs], Article Title: Poly(ADP-ribose) polymerase regulates transient receptor potential channel M2-dependent calpain activation in rd1 mouse retinal degeneration Article Snippet: The sections were rinsed with PBS three times for 10 minutes each and mounted with mounting medium with DAPI (Abcam). .. In some instances TRPM2 was co-labelled with cone photoreceptors using 1h (RT) incubation with fluorescently labeled Article Title: Renal tubular GSDME protects cisplatin nephrotoxicity by impeding OGT-STAT3-S100A7A axis in male mice Article Snippet: .. For renal tubule staining, sections were incubated with 1 μg/mL LTL (FL-1321; detecting of proximal tubules, 1:100), 1 μg/mL Membrane:Article Title: Overexpression of Tn antigen induces chronic pancreatitis in mice. Article Snippet: Proteins were then transferred onto a nitrocellulose membrane (Thermo Fisher). .. The membrane was blocked with 1× Carbo-Free Blocking Solution (Biozol) in TBS-T and incubated overnight with serum diluted 1:20 in TBS-T. Biotinylated lectins, including 10 μg/ml Vicia villosa lectin (VVA, B-1235; Vector Laboratories) and 10 μg/ml Blocking Assay:Article Title: Overexpression of Tn antigen induces chronic pancreatitis in mice. Article Snippet: Proteins were then transferred onto a nitrocellulose membrane (Thermo Fisher). .. The membrane was blocked with 1× Carbo-Free Blocking Solution (Biozol) in TBS-T and incubated overnight with serum diluted 1:20 in TBS-T. Biotinylated lectins, including 10 μg/ml Vicia villosa lectin (VVA, B-1235; Vector Laboratories) and 10 μg/ml Immunodetection:Article Title: Engineering glycosyltransferases into glycan binding proteins using a mammalian surface display platform Article Snippet: Recombinant human ST3Gal1 (rhST3Gal1, Product code: 6905-GT-020), Recombinant human P-selectin Fc chimera (P-selectin-Fc, Product code: 137-PS-050), sheep anti-hGCNT1 IgG (Product code: AF7248-SP) and HRP donkey anti-sheep H + L IgG (Product code: HAF016) were from R&D Systems (Minneapolis, MN). .. Unconjugated Labeling:Article Title: Poly(ADP-ribose) polymerase regulates transient receptor potential channel M2-dependent calpain activation in rd1 mouse retinal degeneration Article Snippet: The sections were rinsed with PBS three times for 10 minutes each and mounted with mounting medium with DAPI (Abcam). .. In some instances TRPM2 was co-labelled with cone photoreceptors using 1h (RT) incubation with fluorescently labeled Staining:Article Title: Renal tubular GSDME protects cisplatin nephrotoxicity by impeding OGT-STAT3-S100A7A axis in male mice Article Snippet: .. For renal tubule staining, sections were incubated with 1 μg/mL LTL (FL-1321; detecting of proximal tubules, 1:100), 1 μg/mL |
