Review



biotinylated peanut agglutinin (pna)  (Vector Laboratories)


Bioz Verified Symbol Vector Laboratories is a verified supplier
Bioz Manufacturer Symbol Vector Laboratories manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 95

    Structured Review

    Vector Laboratories biotinylated peanut agglutinin (pna)
    Biotinylated Peanut Agglutinin (Pna), supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 95/100, based on 912 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/peanut+agglutinin/Biotinylated+Peanut+Agglutinin+(PNA)/custom%40b-1075%4042414656
    Average 95 stars, based on 912 article reviews
    biotinylated peanut agglutinin (pna) - by Bioz Stars, 2026-09
    95/100 stars

    Images

    Related Articles

    Incubation:

    Article Title: Epididymal epithelial cells facilitate NEU1 loading to modulate sperm α-2,6 sialylation, enhance maturation and motility
    Article Snippet: Blocking was conducted with 3% BSA (BIOFROXX) in permeabilization buffer at RT for 1 h with rotation. .. Primary antibodies (anti-NEU1 [1:100, GenScript], anti-NEU3 [1:1000, GenScript], Biotinylated Maackia Amurensis Lectin I [anti-MAL-I, 1:50, Vector Labs], Biotinylated Maackia Amurensis Lectin II [anti-MAL-II, 1:50, Vector Labs], biotinylated Sambucus Nigra Lectin [anti-SNA, 1:50, Vector Labs], biotinylated Wheat Germ Agglutinin [anti-WGA, 1:50, Vector Labs], Peanut Agglutinin [anti-PNA, 1:100], anti-Jacalin [1:300, Vector Labs], Alexa Fluor 594 anti-biotinylated Phosphotyrosine [1:200, Biolegend]), anti-EGFR [1:300, ABclonal], anti-Phospho-EGFR [1:100, ABclonal], JC-1 [1:200, Beyotime], DCFH-DA [1:50, Beyotime] were incubated overnight at 4 °C. .. The next day, samples were centrifuged, washed with PBST, and incubated with secondary antibodies (PE Donkey anti-rabbit IgG [1:1000, Biolegend], FITC Streptavidin [1:500, Biolegend], DyLight 488 Streptavidin [1:500, Biolegend]) in permeabilization buffer at RT for 1 h. After PBST washes, samples were stained with DAPI (Beyotime) and analyzed using a CytoFLEX flow cytometer.

    Article Title: Overexpression of Tn antigen induces chronic pancreatitis in mice.
    Article Snippet: Proteins were then transferred onto a nitrocellulose membrane (Thermo Fisher). .. The membrane was blocked with 1× Carbo-Free Blocking Solution (Biozol) in TBS-T and incubated overnight with serum diluted 1:20 in TBS-T. Biotinylated lectins, including 10 μg/ml Vicia villosa lectin (VVA, B-1235; Vector Laboratories) and 10 μg/ml peanut agglutinin (PNA, B-1075; Vector Laboratories), were complexed with 1 μg of streptavidin-HRP (21126; Pierce, Thermo Fisher Scientific) for detection. .. An antibody against HSPA8 (D12F2; Cell Signaling) was used as a loading control.

    Article Title: Novel mutations in ZMYND15 are associated with male infertility with oligozoospermia/azoospermia.
    Article Snippet: Purpose This study aimed to identify the genetic causes of male infertility associated with oligozoospermia/azoospermia in two unrelated Chinese families.. Methods Whole-exome sequencing (WES) and Sanger sequencing were performed on peripheral blood samples from three infertile individuals with reduced sperm counts.. Semen analysis data were collected, and sperm morphology was evaluated using hematoxylin and eosin staining, along with transmission electron microscopy.

    Article Title: Epididymal epithelial cells facilitate NEU1 loading to modulate sperm α-2,6 sialylation, enhance maturation and motility.
    Article Snippet: Blocking was conducted with 3% BSA (BIOFROXX) in permeabilization buffer at RT for 1 h with rotation. .. Primary antibodies (anti-NEU1 [1:100, GenScript], anti-NEU3 [1:1000, GenScript], Biotinylated Maackia Amurensis Lectin I [anti-MAL-I, 1:50, Vector Labs], Biotinylated Maackia Amurensis Lectin II [anti-MAL-II, 1:50, Vector Labs], biotinylated Sambucus Nigra Lectin [anti-SNA, 1:50, Vector Labs], biotinylated Wheat Germ Agglutinin [anti-WGA, 1:50, Vector Labs], Peanut Agglutinin [antiPNA, 1:100], anti-Jacalin [1:300, Vector Labs], Alexa Fluor 594 anti-biotinylated Phosphotyrosine [1:200, Biolegend]), anti-EGFR [1:300, ABclonal], anti-Phospho-EGFR [1:100, ABclonal], JC-1 [1:200, Beyotime], DCFH-DA [1:50, Beyotime] were incubated overnight at 4 °C. .. The next day, samples were centrifuged, washed with PBST, and incubated with secondary antibodies (PE Donkey anti-rabbit IgG [1:1000, Biolegend], FITC Streptavidin [1:500, Biolegend], DyLight 488 Streptavidin [1:500, Biolegend]) in permeabilization buffer at RT for 1 h. After PBST washes, samples were stained with DAPI (Beyotime) and analyzed using a CytoFLEX flow cytometer.

    Article Title: Poly(ADP-ribose) polymerase regulates transient receptor potential channel M2-dependent calpain activation in rd1 mouse retinal degeneration
    Article Snippet: The sections were rinsed with PBS three times for 10 minutes each and mounted with mounting medium with DAPI (Abcam). .. In some instances TRPM2 was co-labelled with cone photoreceptors using 1h (RT) incubation with fluorescently labeled peanut agglutinin (PNA; 1:500, Ca5# FL1071, Vector Laboratories). .. Microscopy and image analysis in retinal cultures Images of organotypic explant cultures were captured using a Zeiss Imager Z.2 fluorescence microscope, equipped with ApoTome 2, an Axiocam 506 mono camera, and HXP-120V fluorescent lamp (Carl Zeiss).

    Article Title: Renal tubular GSDME protects cisplatin nephrotoxicity by impeding OGT-STAT3-S100A7A axis in male mice
    Article Snippet: .. For renal tubule staining, sections were incubated with 1 μg/mL LTL (FL-1321; detecting of proximal tubules, 1:100), 1 μg/mL Peanut agglutinin (PNA; FL-1071; detecting of distal tubules, 1:100) from Vector Laboratories and Aquaporin 2 (AQP2; sc9882; detecting of collecting ducts, 1:100) from Santa Cruz Biotechnology. .. After PBST (phosphate-buffered saline with 0.1% Tween-20) washing, sections were treated with the respective Alexa Fluor secondary antibody (A-11012, Thermo Fisher).

    Membrane:

    Article Title: Overexpression of Tn antigen induces chronic pancreatitis in mice.
    Article Snippet: Proteins were then transferred onto a nitrocellulose membrane (Thermo Fisher). .. The membrane was blocked with 1× Carbo-Free Blocking Solution (Biozol) in TBS-T and incubated overnight with serum diluted 1:20 in TBS-T. Biotinylated lectins, including 10 μg/ml Vicia villosa lectin (VVA, B-1235; Vector Laboratories) and 10 μg/ml peanut agglutinin (PNA, B-1075; Vector Laboratories), were complexed with 1 μg of streptavidin-HRP (21126; Pierce, Thermo Fisher Scientific) for detection. .. An antibody against HSPA8 (D12F2; Cell Signaling) was used as a loading control.

    Blocking Assay:

    Article Title: Overexpression of Tn antigen induces chronic pancreatitis in mice.
    Article Snippet: Proteins were then transferred onto a nitrocellulose membrane (Thermo Fisher). .. The membrane was blocked with 1× Carbo-Free Blocking Solution (Biozol) in TBS-T and incubated overnight with serum diluted 1:20 in TBS-T. Biotinylated lectins, including 10 μg/ml Vicia villosa lectin (VVA, B-1235; Vector Laboratories) and 10 μg/ml peanut agglutinin (PNA, B-1075; Vector Laboratories), were complexed with 1 μg of streptavidin-HRP (21126; Pierce, Thermo Fisher Scientific) for detection. .. An antibody against HSPA8 (D12F2; Cell Signaling) was used as a loading control.

    Immunodetection:

    Article Title: Engineering glycosyltransferases into glycan binding proteins using a mammalian surface display platform
    Article Snippet: Recombinant human ST3Gal1 (rhST3Gal1, Product code: 6905-GT-020), Recombinant human P-selectin Fc chimera (P-selectin-Fc, Product code: 137-PS-050), sheep anti-hGCNT1 IgG (Product code: AF7248-SP) and HRP donkey anti-sheep H + L IgG (Product code: HAF016) were from R&D Systems (Minneapolis, MN). .. Unconjugated Peanut Agglutinin (PNA, Product code: L-1070), Maackia Amurensis Lectin II (MALII, Product code: L-1260), Erythrina Cristagalli Lectin (ECL, Product code: L-1140), Phaseolus Vulgaris Leucoagglutinin (PHA-L, Product code: L-1110), and H.O.H (Human on Human) Immunodetection kit (Product code: HOH-3000) were from Vector Laboratories (Newark, CA). ..

    Labeling:

    Article Title: Poly(ADP-ribose) polymerase regulates transient receptor potential channel M2-dependent calpain activation in rd1 mouse retinal degeneration
    Article Snippet: The sections were rinsed with PBS three times for 10 minutes each and mounted with mounting medium with DAPI (Abcam). .. In some instances TRPM2 was co-labelled with cone photoreceptors using 1h (RT) incubation with fluorescently labeled peanut agglutinin (PNA; 1:500, Ca5# FL1071, Vector Laboratories). .. Microscopy and image analysis in retinal cultures Images of organotypic explant cultures were captured using a Zeiss Imager Z.2 fluorescence microscope, equipped with ApoTome 2, an Axiocam 506 mono camera, and HXP-120V fluorescent lamp (Carl Zeiss).

    Staining:

    Article Title: Renal tubular GSDME protects cisplatin nephrotoxicity by impeding OGT-STAT3-S100A7A axis in male mice
    Article Snippet: .. For renal tubule staining, sections were incubated with 1 μg/mL LTL (FL-1321; detecting of proximal tubules, 1:100), 1 μg/mL Peanut agglutinin (PNA; FL-1071; detecting of distal tubules, 1:100) from Vector Laboratories and Aquaporin 2 (AQP2; sc9882; detecting of collecting ducts, 1:100) from Santa Cruz Biotechnology. .. After PBST (phosphate-buffered saline with 0.1% Tween-20) washing, sections were treated with the respective Alexa Fluor secondary antibody (A-11012, Thermo Fisher).



    Similar Products

    95
    Vector Laboratories biotinylated peanut agglutinin (pna)
    Biotinylated Peanut Agglutinin (Pna), supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/peanut+agglutinin/Biotinylated+Peanut+Agglutinin+(PNA)/custom%40b-1075%4042414656
    Average 95 stars, based on 1 article reviews
    biotinylated peanut agglutinin (pna) - by Bioz Stars, 2026-09
    95/100 stars
      Buy from Supplier

    94
    Vector Laboratories cy3 labeled peanut agglutinin (pna)
    Cy3 Labeled Peanut Agglutinin (Pna), supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/peanut+agglutinin/Cy3+labeled+Peanut+Agglutinin+(PNA)/custom%40cl-1073%4042314496
    Average 94 stars, based on 1 article reviews
    cy3 labeled peanut agglutinin (pna) - by Bioz Stars, 2026-09
    94/100 stars
      Buy from Supplier

    95
    Vector Laboratories fluorescein peanut agglutinin lectin
    S6K1 activity is required for disease onset and progression in rod Tsc1 −/− mice (A) Top row: representative fundus, <t>fluorescein</t> angiography, and OCT images of an 18-month-old rod Tsc1 −/− S6k1 +/+ mouse with focal RPE atrophy and neovascular pathology (yellow arrows). Bottom row: immunofluorescence and bright field images with red signal from immunofluorescence staining superimposed on bright field of a retinal cross-section of the eye above (section shown in the same orientation as the OCT image) showing loss of RPE65 (red signal) expression in the area of RPE atrophy (dashed line on choroid demarks region of RPE cells loss), indicating a disrupted RPE layer. RPE65 expression with RPE cells is visible on the left third of each panel (area between white arrowheads). Only RPE atrophy is shown on section, not the neovascular pathology. Scale bars: 100 μm; blue, nuclear DAPI; green, peanut <t>agglutinin</t> <t>lectin</t> (PNA) marking cone PR segments; red, RPE65 expression marking RPE cells. (B) Frequency in percentage of phenotypes scored in each genotype at 18 months of age, including microglia activation (white bar), retinal folds (gray bars), focal RPE atrophy (black bars), and neovascular pathologies (green bars). The number of mice examined in each group is indicated in parentheses. Error bar = margin of error (M.O.E.). (C) Representative image of APOE (green signal) accumulation at the RPE/BrM (white arrowheads) in mice with indicated genotype at 12 months of age (4–5 mice were examined in each group). Scale bars: 50 μm; blue, nuclear DAPI; red, peanut agglutinin lectin (PNA) marking cone PR segments; layers in (A and C): RPE, retinal-pigmented epithelium; PS, PR segment region covering inner and outer segments; ONL, outer nuclear layer; INL, inner nuclear layer; GCL, ganglion cell layer; vertical bars in sections mark height of different layers. (D) PR outer segment (POS) clearance in RPE cells of 2-month-old mice is shown as percentage of POS remaining at 11 am when compared to 8 am in genotypes indicated ( N = 4–8 RPE flat mounts/genotype). (E) Percentage of di-DHA PE (left) and PC (right) phospholipids as a total of PE (left) and PC (right) phospholipids in genotypes indicated ( N = 5–6 retinas/genotype). Results in (D and E) are shown as mean ± S.E.M. (∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗∗ p < 0.0001; n.s., not significant).
    Fluorescein Peanut Agglutinin Lectin, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/peanut+agglutinin/Fluorescein+labeled+Peanut+Agglutinin+(PNA)/pmc12996783-301-17-22
    Average 95 stars, based on 1 article reviews
    fluorescein peanut agglutinin lectin - by Bioz Stars, 2026-09
    95/100 stars
      Buy from Supplier

    95
    Vector Laboratories fluorescein labeled peanut agglutinin (pna)
    S6K1 activity is required for disease onset and progression in rod Tsc1 −/− mice (A) Top row: representative fundus, <t>fluorescein</t> angiography, and OCT images of an 18-month-old rod Tsc1 −/− S6k1 +/+ mouse with focal RPE atrophy and neovascular pathology (yellow arrows). Bottom row: immunofluorescence and bright field images with red signal from immunofluorescence staining superimposed on bright field of a retinal cross-section of the eye above (section shown in the same orientation as the OCT image) showing loss of RPE65 (red signal) expression in the area of RPE atrophy (dashed line on choroid demarks region of RPE cells loss), indicating a disrupted RPE layer. RPE65 expression with RPE cells is visible on the left third of each panel (area between white arrowheads). Only RPE atrophy is shown on section, not the neovascular pathology. Scale bars: 100 μm; blue, nuclear DAPI; green, peanut <t>agglutinin</t> <t>lectin</t> (PNA) marking cone PR segments; red, RPE65 expression marking RPE cells. (B) Frequency in percentage of phenotypes scored in each genotype at 18 months of age, including microglia activation (white bar), retinal folds (gray bars), focal RPE atrophy (black bars), and neovascular pathologies (green bars). The number of mice examined in each group is indicated in parentheses. Error bar = margin of error (M.O.E.). (C) Representative image of APOE (green signal) accumulation at the RPE/BrM (white arrowheads) in mice with indicated genotype at 12 months of age (4–5 mice were examined in each group). Scale bars: 50 μm; blue, nuclear DAPI; red, peanut agglutinin lectin (PNA) marking cone PR segments; layers in (A and C): RPE, retinal-pigmented epithelium; PS, PR segment region covering inner and outer segments; ONL, outer nuclear layer; INL, inner nuclear layer; GCL, ganglion cell layer; vertical bars in sections mark height of different layers. (D) PR outer segment (POS) clearance in RPE cells of 2-month-old mice is shown as percentage of POS remaining at 11 am when compared to 8 am in genotypes indicated ( N = 4–8 RPE flat mounts/genotype). (E) Percentage of di-DHA PE (left) and PC (right) phospholipids as a total of PE (left) and PC (right) phospholipids in genotypes indicated ( N = 5–6 retinas/genotype). Results in (D and E) are shown as mean ± S.E.M. (∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗∗ p < 0.0001; n.s., not significant).
    Fluorescein Labeled Peanut Agglutinin (Pna), supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/peanut+agglutinin/Fluorescein+labeled+Peanut+Agglutinin+(PNA)/custom%40fl-1071%4041858838
    Average 95 stars, based on 1 article reviews
    fluorescein labeled peanut agglutinin (pna) - by Bioz Stars, 2026-09
    95/100 stars
      Buy from Supplier

    95
    Vector Laboratories unconjugated peanut agglutinin (pna)
    S6K1 activity is required for disease onset and progression in rod Tsc1 −/− mice (A) Top row: representative fundus, <t>fluorescein</t> angiography, and OCT images of an 18-month-old rod Tsc1 −/− S6k1 +/+ mouse with focal RPE atrophy and neovascular pathology (yellow arrows). Bottom row: immunofluorescence and bright field images with red signal from immunofluorescence staining superimposed on bright field of a retinal cross-section of the eye above (section shown in the same orientation as the OCT image) showing loss of RPE65 (red signal) expression in the area of RPE atrophy (dashed line on choroid demarks region of RPE cells loss), indicating a disrupted RPE layer. RPE65 expression with RPE cells is visible on the left third of each panel (area between white arrowheads). Only RPE atrophy is shown on section, not the neovascular pathology. Scale bars: 100 μm; blue, nuclear DAPI; green, peanut <t>agglutinin</t> <t>lectin</t> (PNA) marking cone PR segments; red, RPE65 expression marking RPE cells. (B) Frequency in percentage of phenotypes scored in each genotype at 18 months of age, including microglia activation (white bar), retinal folds (gray bars), focal RPE atrophy (black bars), and neovascular pathologies (green bars). The number of mice examined in each group is indicated in parentheses. Error bar = margin of error (M.O.E.). (C) Representative image of APOE (green signal) accumulation at the RPE/BrM (white arrowheads) in mice with indicated genotype at 12 months of age (4–5 mice were examined in each group). Scale bars: 50 μm; blue, nuclear DAPI; red, peanut agglutinin lectin (PNA) marking cone PR segments; layers in (A and C): RPE, retinal-pigmented epithelium; PS, PR segment region covering inner and outer segments; ONL, outer nuclear layer; INL, inner nuclear layer; GCL, ganglion cell layer; vertical bars in sections mark height of different layers. (D) PR outer segment (POS) clearance in RPE cells of 2-month-old mice is shown as percentage of POS remaining at 11 am when compared to 8 am in genotypes indicated ( N = 4–8 RPE flat mounts/genotype). (E) Percentage of di-DHA PE (left) and PC (right) phospholipids as a total of PE (left) and PC (right) phospholipids in genotypes indicated ( N = 5–6 retinas/genotype). Results in (D and E) are shown as mean ± S.E.M. (∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗∗ p < 0.0001; n.s., not significant).
    Unconjugated Peanut Agglutinin (Pna), supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/peanut+agglutinin/Unconjugated+Peanut+Agglutinin+(PNA)/custom%40l-1070%4042134327
    Average 95 stars, based on 1 article reviews
    unconjugated peanut agglutinin (pna) - by Bioz Stars, 2026-09
    95/100 stars
      Buy from Supplier

    86
    Eurobio peanut agglutinin pna fitc
    S6K1 activity is required for disease onset and progression in rod Tsc1 −/− mice (A) Top row: representative fundus, <t>fluorescein</t> angiography, and OCT images of an 18-month-old rod Tsc1 −/− S6k1 +/+ mouse with focal RPE atrophy and neovascular pathology (yellow arrows). Bottom row: immunofluorescence and bright field images with red signal from immunofluorescence staining superimposed on bright field of a retinal cross-section of the eye above (section shown in the same orientation as the OCT image) showing loss of RPE65 (red signal) expression in the area of RPE atrophy (dashed line on choroid demarks region of RPE cells loss), indicating a disrupted RPE layer. RPE65 expression with RPE cells is visible on the left third of each panel (area between white arrowheads). Only RPE atrophy is shown on section, not the neovascular pathology. Scale bars: 100 μm; blue, nuclear DAPI; green, peanut <t>agglutinin</t> <t>lectin</t> (PNA) marking cone PR segments; red, RPE65 expression marking RPE cells. (B) Frequency in percentage of phenotypes scored in each genotype at 18 months of age, including microglia activation (white bar), retinal folds (gray bars), focal RPE atrophy (black bars), and neovascular pathologies (green bars). The number of mice examined in each group is indicated in parentheses. Error bar = margin of error (M.O.E.). (C) Representative image of APOE (green signal) accumulation at the RPE/BrM (white arrowheads) in mice with indicated genotype at 12 months of age (4–5 mice were examined in each group). Scale bars: 50 μm; blue, nuclear DAPI; red, peanut agglutinin lectin (PNA) marking cone PR segments; layers in (A and C): RPE, retinal-pigmented epithelium; PS, PR segment region covering inner and outer segments; ONL, outer nuclear layer; INL, inner nuclear layer; GCL, ganglion cell layer; vertical bars in sections mark height of different layers. (D) PR outer segment (POS) clearance in RPE cells of 2-month-old mice is shown as percentage of POS remaining at 11 am when compared to 8 am in genotypes indicated ( N = 4–8 RPE flat mounts/genotype). (E) Percentage of di-DHA PE (left) and PC (right) phospholipids as a total of PE (left) and PC (right) phospholipids in genotypes indicated ( N = 5–6 retinas/genotype). Results in (D and E) are shown as mean ± S.E.M. (∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗∗ p < 0.0001; n.s., not significant).
    Peanut Agglutinin Pna Fitc, supplied by Eurobio, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/peanut+agglutinin/agglutinin+fitc+peanut+pna/pm42085930-162-35-38
    Average 86 stars, based on 1 article reviews
    peanut agglutinin pna fitc - by Bioz Stars, 2026-09
    86/100 stars
      Buy from Supplier

    95
    Vector Laboratories control lectins
    S6K1 activity is required for disease onset and progression in rod Tsc1 −/− mice (A) Top row: representative fundus, <t>fluorescein</t> angiography, and OCT images of an 18-month-old rod Tsc1 −/− S6k1 +/+ mouse with focal RPE atrophy and neovascular pathology (yellow arrows). Bottom row: immunofluorescence and bright field images with red signal from immunofluorescence staining superimposed on bright field of a retinal cross-section of the eye above (section shown in the same orientation as the OCT image) showing loss of RPE65 (red signal) expression in the area of RPE atrophy (dashed line on choroid demarks region of RPE cells loss), indicating a disrupted RPE layer. RPE65 expression with RPE cells is visible on the left third of each panel (area between white arrowheads). Only RPE atrophy is shown on section, not the neovascular pathology. Scale bars: 100 μm; blue, nuclear DAPI; green, peanut <t>agglutinin</t> <t>lectin</t> (PNA) marking cone PR segments; red, RPE65 expression marking RPE cells. (B) Frequency in percentage of phenotypes scored in each genotype at 18 months of age, including microglia activation (white bar), retinal folds (gray bars), focal RPE atrophy (black bars), and neovascular pathologies (green bars). The number of mice examined in each group is indicated in parentheses. Error bar = margin of error (M.O.E.). (C) Representative image of APOE (green signal) accumulation at the RPE/BrM (white arrowheads) in mice with indicated genotype at 12 months of age (4–5 mice were examined in each group). Scale bars: 50 μm; blue, nuclear DAPI; red, peanut agglutinin lectin (PNA) marking cone PR segments; layers in (A and C): RPE, retinal-pigmented epithelium; PS, PR segment region covering inner and outer segments; ONL, outer nuclear layer; INL, inner nuclear layer; GCL, ganglion cell layer; vertical bars in sections mark height of different layers. (D) PR outer segment (POS) clearance in RPE cells of 2-month-old mice is shown as percentage of POS remaining at 11 am when compared to 8 am in genotypes indicated ( N = 4–8 RPE flat mounts/genotype). (E) Percentage of di-DHA PE (left) and PC (right) phospholipids as a total of PE (left) and PC (right) phospholipids in genotypes indicated ( N = 5–6 retinas/genotype). Results in (D and E) are shown as mean ± S.E.M. (∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗∗ p < 0.0001; n.s., not significant).
    Control Lectins, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/peanut+agglutinin/Biotinylated+Peanut+Agglutinin+(PNA)/pm42037405-280-0-17
    Average 95 stars, based on 1 article reviews
    control lectins - by Bioz Stars, 2026-09
    95/100 stars
      Buy from Supplier

    95
    Vector Laboratories fitc pna fl 1071
    S6K1 activity is required for disease onset and progression in rod Tsc1 −/− mice (A) Top row: representative fundus, <t>fluorescein</t> angiography, and OCT images of an 18-month-old rod Tsc1 −/− S6k1 +/+ mouse with focal RPE atrophy and neovascular pathology (yellow arrows). Bottom row: immunofluorescence and bright field images with red signal from immunofluorescence staining superimposed on bright field of a retinal cross-section of the eye above (section shown in the same orientation as the OCT image) showing loss of RPE65 (red signal) expression in the area of RPE atrophy (dashed line on choroid demarks region of RPE cells loss), indicating a disrupted RPE layer. RPE65 expression with RPE cells is visible on the left third of each panel (area between white arrowheads). Only RPE atrophy is shown on section, not the neovascular pathology. Scale bars: 100 μm; blue, nuclear DAPI; green, peanut <t>agglutinin</t> <t>lectin</t> (PNA) marking cone PR segments; red, RPE65 expression marking RPE cells. (B) Frequency in percentage of phenotypes scored in each genotype at 18 months of age, including microglia activation (white bar), retinal folds (gray bars), focal RPE atrophy (black bars), and neovascular pathologies (green bars). The number of mice examined in each group is indicated in parentheses. Error bar = margin of error (M.O.E.). (C) Representative image of APOE (green signal) accumulation at the RPE/BrM (white arrowheads) in mice with indicated genotype at 12 months of age (4–5 mice were examined in each group). Scale bars: 50 μm; blue, nuclear DAPI; red, peanut agglutinin lectin (PNA) marking cone PR segments; layers in (A and C): RPE, retinal-pigmented epithelium; PS, PR segment region covering inner and outer segments; ONL, outer nuclear layer; INL, inner nuclear layer; GCL, ganglion cell layer; vertical bars in sections mark height of different layers. (D) PR outer segment (POS) clearance in RPE cells of 2-month-old mice is shown as percentage of POS remaining at 11 am when compared to 8 am in genotypes indicated ( N = 4–8 RPE flat mounts/genotype). (E) Percentage of di-DHA PE (left) and PC (right) phospholipids as a total of PE (left) and PC (right) phospholipids in genotypes indicated ( N = 5–6 retinas/genotype). Results in (D and E) are shown as mean ± S.E.M. (∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗∗ p < 0.0001; n.s., not significant).
    Fitc Pna Fl 1071, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/peanut+agglutinin/Fluorescein+labeled+Peanut+Agglutinin+(PNA)/pm42034626-583-0-16
    Average 95 stars, based on 1 article reviews
    fitc pna fl 1071 - by Bioz Stars, 2026-09
    95/100 stars
      Buy from Supplier

    Image Search Results


    S6K1 activity is required for disease onset and progression in rod Tsc1 −/− mice (A) Top row: representative fundus, fluorescein angiography, and OCT images of an 18-month-old rod Tsc1 −/− S6k1 +/+ mouse with focal RPE atrophy and neovascular pathology (yellow arrows). Bottom row: immunofluorescence and bright field images with red signal from immunofluorescence staining superimposed on bright field of a retinal cross-section of the eye above (section shown in the same orientation as the OCT image) showing loss of RPE65 (red signal) expression in the area of RPE atrophy (dashed line on choroid demarks region of RPE cells loss), indicating a disrupted RPE layer. RPE65 expression with RPE cells is visible on the left third of each panel (area between white arrowheads). Only RPE atrophy is shown on section, not the neovascular pathology. Scale bars: 100 μm; blue, nuclear DAPI; green, peanut agglutinin lectin (PNA) marking cone PR segments; red, RPE65 expression marking RPE cells. (B) Frequency in percentage of phenotypes scored in each genotype at 18 months of age, including microglia activation (white bar), retinal folds (gray bars), focal RPE atrophy (black bars), and neovascular pathologies (green bars). The number of mice examined in each group is indicated in parentheses. Error bar = margin of error (M.O.E.). (C) Representative image of APOE (green signal) accumulation at the RPE/BrM (white arrowheads) in mice with indicated genotype at 12 months of age (4–5 mice were examined in each group). Scale bars: 50 μm; blue, nuclear DAPI; red, peanut agglutinin lectin (PNA) marking cone PR segments; layers in (A and C): RPE, retinal-pigmented epithelium; PS, PR segment region covering inner and outer segments; ONL, outer nuclear layer; INL, inner nuclear layer; GCL, ganglion cell layer; vertical bars in sections mark height of different layers. (D) PR outer segment (POS) clearance in RPE cells of 2-month-old mice is shown as percentage of POS remaining at 11 am when compared to 8 am in genotypes indicated ( N = 4–8 RPE flat mounts/genotype). (E) Percentage of di-DHA PE (left) and PC (right) phospholipids as a total of PE (left) and PC (right) phospholipids in genotypes indicated ( N = 5–6 retinas/genotype). Results in (D and E) are shown as mean ± S.E.M. (∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗∗ p < 0.0001; n.s., not significant).

    Journal: Molecular Therapy. Nucleic Acids

    Article Title: An siRNA targeting S6k1 identifies photoreceptor phospholipid metabolism as a contributor to lipid buildup in age-related macular degeneration

    doi: 10.1016/j.omtn.2026.102878

    Figure Lengend Snippet: S6K1 activity is required for disease onset and progression in rod Tsc1 −/− mice (A) Top row: representative fundus, fluorescein angiography, and OCT images of an 18-month-old rod Tsc1 −/− S6k1 +/+ mouse with focal RPE atrophy and neovascular pathology (yellow arrows). Bottom row: immunofluorescence and bright field images with red signal from immunofluorescence staining superimposed on bright field of a retinal cross-section of the eye above (section shown in the same orientation as the OCT image) showing loss of RPE65 (red signal) expression in the area of RPE atrophy (dashed line on choroid demarks region of RPE cells loss), indicating a disrupted RPE layer. RPE65 expression with RPE cells is visible on the left third of each panel (area between white arrowheads). Only RPE atrophy is shown on section, not the neovascular pathology. Scale bars: 100 μm; blue, nuclear DAPI; green, peanut agglutinin lectin (PNA) marking cone PR segments; red, RPE65 expression marking RPE cells. (B) Frequency in percentage of phenotypes scored in each genotype at 18 months of age, including microglia activation (white bar), retinal folds (gray bars), focal RPE atrophy (black bars), and neovascular pathologies (green bars). The number of mice examined in each group is indicated in parentheses. Error bar = margin of error (M.O.E.). (C) Representative image of APOE (green signal) accumulation at the RPE/BrM (white arrowheads) in mice with indicated genotype at 12 months of age (4–5 mice were examined in each group). Scale bars: 50 μm; blue, nuclear DAPI; red, peanut agglutinin lectin (PNA) marking cone PR segments; layers in (A and C): RPE, retinal-pigmented epithelium; PS, PR segment region covering inner and outer segments; ONL, outer nuclear layer; INL, inner nuclear layer; GCL, ganglion cell layer; vertical bars in sections mark height of different layers. (D) PR outer segment (POS) clearance in RPE cells of 2-month-old mice is shown as percentage of POS remaining at 11 am when compared to 8 am in genotypes indicated ( N = 4–8 RPE flat mounts/genotype). (E) Percentage of di-DHA PE (left) and PC (right) phospholipids as a total of PE (left) and PC (right) phospholipids in genotypes indicated ( N = 5–6 retinas/genotype). Results in (D and E) are shown as mean ± S.E.M. (∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗∗ p < 0.0001; n.s., not significant).

    Article Snippet: The following reagents already had a chromophore conjugated: rhodamine phalloidin (Life Technology, Cat. #: R415; 1:100) and fluorescein peanut agglutinin lectin (PNA; Vector Laboratories, Cat. #: FL-1071; 1:500).

    Techniques: Activity Assay, Immunofluorescence, Staining, Expressing, Activation Assay

    S6k1 silencing in mouse reverses early disease pathologies in rod Tsc1 −/− mice (A and B) Long-term siRNA retention and silencing efficacy in rod Tsc1 −/− mice examined at 3, 6, and 9 months post-injection. Mice received one intravitreal injection of 15 μg of siRNA reagents at 3 months of age. (A) Distribution of tetra-siRNA S6k1 and S6K1 protein expression in rod Tsc1 −/− mouse retinas. Left: tiled retinal sections showing either tetra-siRNA NTC (top) or tetra-siRNA S6k1 (bottom, visualized with RNAScope, red signal) at 3 months post-injection (scale bars: 500 μm). Right: higher magnification of tetra-siRNA S6k1 distribution on retinal sections and S6K1 protein expression at time points indicated. Tetra-siRNA S6k1 is visualized with RNAScope (red signal), and S6K1 protein expression is visualized by immunohistochemistry (purple signal). Staining for tetra-siRNA S6k1 and S6K1 was performed on separate slides. Scale bars: 50 μm; PS, PR segment region covering inner and outer segments; ONL, outer nuclear layer; INL, inner nuclear layer; GCL, ganglion cell layer; vertical bars in sections mark height of different layers. (B) Silencing efficiency of tetra-siRNA S6k1 (blue bars) at time points indicated post-intravitreal injection when compared to the NTC (green bars). Silencing was measured by western blotting with retinal protein extracts. Mice were all injected at 3 months of age ( N = 5–8 retinas/group). (C) Percentage silencing in PR vs. non-PR cells that were enriched by FACS at 2 months post-intravitreal delivery of siRNA. The percentage of protein expression level is normalized to the tetra-siRNA NTC treated group. (D) PR outer segment (POS) clearance in RPE cells of 4-month-old mice shown as percentage of POS remaining at 11 am when compared to the peak of shedding at 8 am in the genotypes indicated. rod Tsc1 −/− mice were injected at 2 months of age with siRNA reagents indicated ( N = 4–7 eyes/group). (E and F) Reversal of APOE accumulation at the BrM in tetra-siRNA S6k1 -treated mice. (E) APOE protein expression level measured by western blotting with RPE/choroid protein extracts of 15-month-old rod Tsc1 −/− mice that are untreated or treated with either tetra-siRNA NTC or tetra-siRNA S6k1 for 3 months (treatment started at 12 months of age). Expression levels are compared to 15-month-old littermate control rod Tsc1 +/+ mice ( N = 5–10 eyes/group). (B–E) Results are shown as mean ± S.E.M. Each dot represents one retina or RPE/choroid from one mouse. Only one eye per mouse was used for each analysis (∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001; ∗∗∗∗ p < 0.0001; green bars represent tetra-siRNA NTC and blue bars tetra-siRNA S6k1 -injected eyes). (F) Retinal cross-section of rod Tsc1 −/− eyes showing reduction in the accumulation APOE (green signal) at the BrM (white arrowheads) of tetra-siRNA S6k1 -injected eyes (right panel). Mice were injected at 12 months of age and analyzed 3 months post-injection. Scale bars: 50 μm; blue, nuclear DAPI; red, peanut agglutinin lectin (PNA) marking cone PR segments; RPE, retinal-pigmented epithelium; PS, PR segment region covering inner and outer segments; ONL, outer nuclear layer; INL, inner nuclear layer; GCL, ganglion cell layer; vertical bars in sections mark height of different layers.

    Journal: Molecular Therapy. Nucleic Acids

    Article Title: An siRNA targeting S6k1 identifies photoreceptor phospholipid metabolism as a contributor to lipid buildup in age-related macular degeneration

    doi: 10.1016/j.omtn.2026.102878

    Figure Lengend Snippet: S6k1 silencing in mouse reverses early disease pathologies in rod Tsc1 −/− mice (A and B) Long-term siRNA retention and silencing efficacy in rod Tsc1 −/− mice examined at 3, 6, and 9 months post-injection. Mice received one intravitreal injection of 15 μg of siRNA reagents at 3 months of age. (A) Distribution of tetra-siRNA S6k1 and S6K1 protein expression in rod Tsc1 −/− mouse retinas. Left: tiled retinal sections showing either tetra-siRNA NTC (top) or tetra-siRNA S6k1 (bottom, visualized with RNAScope, red signal) at 3 months post-injection (scale bars: 500 μm). Right: higher magnification of tetra-siRNA S6k1 distribution on retinal sections and S6K1 protein expression at time points indicated. Tetra-siRNA S6k1 is visualized with RNAScope (red signal), and S6K1 protein expression is visualized by immunohistochemistry (purple signal). Staining for tetra-siRNA S6k1 and S6K1 was performed on separate slides. Scale bars: 50 μm; PS, PR segment region covering inner and outer segments; ONL, outer nuclear layer; INL, inner nuclear layer; GCL, ganglion cell layer; vertical bars in sections mark height of different layers. (B) Silencing efficiency of tetra-siRNA S6k1 (blue bars) at time points indicated post-intravitreal injection when compared to the NTC (green bars). Silencing was measured by western blotting with retinal protein extracts. Mice were all injected at 3 months of age ( N = 5–8 retinas/group). (C) Percentage silencing in PR vs. non-PR cells that were enriched by FACS at 2 months post-intravitreal delivery of siRNA. The percentage of protein expression level is normalized to the tetra-siRNA NTC treated group. (D) PR outer segment (POS) clearance in RPE cells of 4-month-old mice shown as percentage of POS remaining at 11 am when compared to the peak of shedding at 8 am in the genotypes indicated. rod Tsc1 −/− mice were injected at 2 months of age with siRNA reagents indicated ( N = 4–7 eyes/group). (E and F) Reversal of APOE accumulation at the BrM in tetra-siRNA S6k1 -treated mice. (E) APOE protein expression level measured by western blotting with RPE/choroid protein extracts of 15-month-old rod Tsc1 −/− mice that are untreated or treated with either tetra-siRNA NTC or tetra-siRNA S6k1 for 3 months (treatment started at 12 months of age). Expression levels are compared to 15-month-old littermate control rod Tsc1 +/+ mice ( N = 5–10 eyes/group). (B–E) Results are shown as mean ± S.E.M. Each dot represents one retina or RPE/choroid from one mouse. Only one eye per mouse was used for each analysis (∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001; ∗∗∗∗ p < 0.0001; green bars represent tetra-siRNA NTC and blue bars tetra-siRNA S6k1 -injected eyes). (F) Retinal cross-section of rod Tsc1 −/− eyes showing reduction in the accumulation APOE (green signal) at the BrM (white arrowheads) of tetra-siRNA S6k1 -injected eyes (right panel). Mice were injected at 12 months of age and analyzed 3 months post-injection. Scale bars: 50 μm; blue, nuclear DAPI; red, peanut agglutinin lectin (PNA) marking cone PR segments; RPE, retinal-pigmented epithelium; PS, PR segment region covering inner and outer segments; ONL, outer nuclear layer; INL, inner nuclear layer; GCL, ganglion cell layer; vertical bars in sections mark height of different layers.

    Article Snippet: The following reagents already had a chromophore conjugated: rhodamine phalloidin (Life Technology, Cat. #: R415; 1:100) and fluorescein peanut agglutinin lectin (PNA; Vector Laboratories, Cat. #: FL-1071; 1:500).

    Techniques: Injection, Expressing, RNAscope, Immunohistochemistry, Staining, Western Blot, Control